When analyzing posttranslational modifications and topdown sequencing of biomolecules are all important, electron transfer dissociation etd provides a. Product performance, product reliability, product design. You can enter more than one fragment ion under fragments. Telephone and fax from the usa or canada, phone 800 252hplc, or fax 508 872 1990. How to test for gc transfer line air leaks or source insulator problems on the autospec wkb64335. Synapt g2s mass spectrometer overview and maintenance guide. Waters synapt g2 operators, overview and maintenance manual. Synapt g2 s i is the only way to combine three dimensions of resolution, to access unique levels of separation, selectivity, sensitivity and structural insight. Designed for leading researchers working at the limits of conventional mass spectrometery capabilities who need to further characterize and define their samples, waters synapt tm hdms tm system offers unique, enabling functionality waters synapt hdms tm system enables analysis of samples differentiated by size and shape and mass, to deliver.
On the tune page, from the setup dropdown menu, select system view. Designed for leading researchers working at the limits of conventional mass spectrometery capabilities who need to further characterize and define their samples, waters synapt tm hdms tm system offers unique, enabling functionality. Waters acquity uplc leak sensor maintenance instructions part number. Wait for the epc to boot the flashing whitepurple light on the synapt g2 ssi will stop flashing and will remain solid whitepurple. Waters designed the synapt g2s mass spectrometer to be used as a research tool to. A locking handle locks the sliding platform, preventing its unintentional movement when you disconnect the locking ring from the apgc source enclosure. To do this, go to the tune page and select view top dropdown menu second from the left. For unparalleled discovery power and versatility, look no further.
The waters synapt g2si system is utilized for protein applications such as proteinprotein interaction experiments. Waters introduces synapt g2s mass spectrometer, combining. Download waters synapt g2 operators, overview and maintenance manual. The waters synapt g2 ms is compatible with the acquity uplc and. Like the g2 this instrument has been modified with a custom surfaceinduced dissociation sid device which can be placed before the im region sidim, after the im region imsid, or on either side of the im cell to allow for sidimsid. Instrumentation laboratory for biological mass spectrometry. All voltages are relative to the transfer cell voltage, which is controlled by the static.
This is a high mass accuracy mass spectrometer equipped with an ion mobility cell. How to make sodium formate for qtof calibrations waters. Trap twave, ims twave, transfer twave stepwavetm ion optics video overview of the stepwavetm technology. For other locations worldwide, phone and fax numbers appear in the waters web site. Novel technology provides cutting edge performance.
Why we need such feedback is that we are currently considering to buy a qtof system and we will place a tender open competition to provide the best system from waters, agilent, bruker, sciex, and leco. Waters strongly recommend installation and operation confirmationtraining by a field. Restore the last known good settings from pm backup. We know the theoretical aspects and specifications of the system. Mar 29, 2010 synapt g2s i is equipped with a larger ion sampling orifice, an enhanced vacuum pumping configuration and revolutionary stepwave ion transfer optics.
Waters introduces synapt g2s mass spectrometer lab manager. This groundbreaking dualtwave, offaxis design transfers ions from the ion source to the quadrupole ms analyzer with the highest possible efficiency, at the same time. Weve combined quantof breakthrough quantitative tof technology and enhanced high definition ms technologies to provide you with intuitive operation, application flexibility and a. Transforming your capacity for discovery, understanding, and success. Synapt g2 only trolley cabinet locking lever locking plate stabilizers the apgc trolley is a mobile steel cabinet on top of which the apgc. Observe the ip address listed for the synapt g2s i mshdms which will have the format 192.
Waters apgc operators manual supplement pdf download. View online or download waters xevo g2 xs qtof overview and maintenance manual. In addition to standard collision induced dissociation cid, this instrument can also conduct fragmentation via electron transfer dissociation etd. Development is being supported by nih sbir grant 1r43gm479201. The second generation synapt g2 platform from waters provides a new dimension of performance to drive scientific discovery like never before. The synapt g2 s incorporates both highsensitivity waters stepwavetm ion transfer optics and triwave ion mobility technologies along with a suite of new informatics tools to take qualitative and quantitative high resolution performance to a new level. See the xevo g2 qtof in action and learn how you can obtain the highest quality data and the most comprehensive information. Features quantitative quadrupole timeofflight mass spectrometer quantoftm high resolution exact msms travelling wave ion mobility separation triwavetm cell, incl. S1 diagram of the rfconfining drift cell integrated into the waters synapt g2 hdms. To access the highest levels of information content from your most analytically challenging samples, or utilize analytical tools to make scientific discoveries not possible by any other means, look no further than synapt g2 s.
How to tune up the resolution on a qtof wkb440 waters. Synapt g2 s combines revolutionary high performance stepwave ion optics, quantitative tof. Tof g2 s sample kit 1 700008842 the tof g2 s sample kit 1 is intended for the verification of system performance for tof g2 s. Developments in data independent ms acquisition comprehensive. Electron transfer dissociation provides higherorder.
Synapt g1 high definition mass spectrometer duke gcb. Tuning a high transmission ion guide to prevent gasphase proton. Synapt g2 laboratory equipment pdf manual download. Click view vacuum, and then go to the vacuum tab and ensure that you have real vacuum readings not 1e8 for the tof vacuum. Mar 29, 2010 synapt g2 s is equipped with a larger ion sampling orifice, an enhanced vacuum pumping configuration and revolutionary stepwave ion transfer optics. The ability of synapt g2 to deliver high mass accuracy and accu. A newer generation of waters synapt g2 instrument with improved stepwave design after the ion source for improved sensitivity. Waters synapt g2si high definition mass spectrometry center. Waters synapt g2si high definition synapt g2si mass spectrometer, with a mass range up to 100,000 on mz scale in resolution mode, provides unique separation capability of highly complex samples in addition to hplc by its advanced twave ionmobility technology. See inside the synapt g2 mass spectrometer from waters corp. How to do an electronic reboot on a synapt or qtof. The synapt g2 s incorporates both highsensitivity waters stepwave ion transfer optics and triwave ion mobility technologies along with a suite of new informatics tools to take qualitative and quantitative high resolution performance to a new level.
The synapt g2 s also features waters universal ion source architecture engineered to take maximum advantage of uplc allowing the widest range of waters ionization sources including. View and download waters synapt g2 operators, overview and maintenance manual online. Synapt g2si ms provides the most uncompromising high resolution. You can use the trap twave and transfer twave regions of the triwave device for. Electronic supplementary information the royal society of. Synapt g2s high definition mass spectrometer from waters. Synapt g2s hdms waters the mclean group laboratory.
Laboratory equipment waters 2410 service manual 56 pages. Hi colleagues, we realy do want some feedback from users of synapt g2 hdms system. Waters contact information contacting medium information internet the waters web site includes contact information for waters locations worldwide. On xevo instruments, flip only the bottom toggle switch at the bottom in the middle of the back of the xevo. Poweroff the ms breakers on the back starting with the epc, then electronics, then vacuum on synapt, qtof p or lct p instruments. Synapt g2s hdms waters the mclean group laboratory for. The exd ws250 option for waters synapt ms is in the early adopter phase of development.
For synapt g2si, trap and transfer collision energy. I got a problem with my instrument uplcms waters qtof g2. Careful transfer of ions into the gasphase permits the measurement of protein structures, with. By combining stepwave plus quantof and triwave technologies, it provides a step change in ms performance. Hi everybody, where could i find a stepbystep user manual for waters lcms xevo g2 xs. Dissociation cid andor electron transfer dissociation etd fragmentation with. Using collision cross section distributions to assess. Move the sprayer back and to the lefthand side until the tip aligns with the groove.
The platform carries the heated transfer line and the gc instrument, which the mass spectrometer connects to via the apgc source enclosure and ion chamber. The instrument incorporates the newest technologies in the area of ion optics and ion mobility separation, with interchangeable universal ion source architecture, allowing. The desolvated compounds are ionized by charge transfer as they pass through a plasma created by a corona discharge. Here, we investigate this approach using topdown etd of tetrameric hemoglobin, concanavalin a, and alcohol dehydrogenase combined with ion mobility im on a commercially available quadrupoleion mobilitytimeofflight instrument waters synapt g2. Set a collision energy ramp by entering the low and high collision energy in the trap or, if present, transfer ce ramp windows. Waters introduces xevo g2xs qtof and xevo tqs micro mass. Synapt g2 ms with quantof technology and high definition. L of deionized or lcmsgrade water to the 20ml volumetric flask. Turn off all gasses and take a screen capture of the vacuum view to record the vacuum you had before you vented.
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